#Editseq module of dnastar software#

In addition, CgDM9CP-4-positive cells were found to be much more abundant in hemocytes from gill than that from hemolymph, with most of them located in the gill filament. The mRNA expression level of CgDM9CP-4 in hemocytes was decreased after RNAi of hematopoietic-related factors ( CgGATA, CgRunt, CgSCL, and CgNotch).

The reactive oxygen species (ROS) and the expression levels of cytokines in CgDM9CP-4-positive hemocytes were much lower than that in CgDM9CP-4-negative hemocytes. CgDM9CP-4 was highly expressed in non-phagocytes (~82% of total hemocytes). Flow cytometry analysis demonstrated that the CgDM9CP-4-positive hemocytes accounted for 7.3% of the total hemocytes, and they were small in size and less in granularity.

CgDM9CP-4 was mostly expressed in gill and some of the hemocytes. anguillarum, Escherichia coli, Pichia pastoris and Yarrowia lipolytica). The recombinant protein of CgDM9CP-4 (r CgDM9CP-4) displayed a broad binding spectrum towards various pathogen-associated molecular patterns (PAMPs) (LPS, PGN, ОІ-glucan and Mannose) and microorganisms ( Staphylococcus aureus, Micrococcus luteus, V. The transcripts level of CgDM9CP-4 in circulating hemocytes were decreased after LPS, PGN and Vibrio splendidus stimulations. CgDM9CP-4, about 16 kDa with only two tandem DM9 domains, was highly enriched in hemocytes and gill. In the present study, a novel member of DM9 domain containing protein (designated as CgDM9CP-4) was identified from C. Two proteins with DM9 repeats have been recently identified from Pacific oyster Crassostrea gigas as mannose-specific binding pattern-recognition receptors (PRRs).

5Southern Laboratory of Ocean Science and Engineering (Guangdong, Zhuhai), Zhuhai, ChinaДM9 refers to an uncharacterized protein domain that is originally discovered in Drosophila melanogaster.

4Functional Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.

3Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China.

2Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

1Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China.

What part of the sequence is vector and what part is insert (1-x =vector, y-z = insert)C) Translate the insert in all 3 frames.Zhihao Jia 1,2, Shuai Jiang 2, Mengqiang Wang 2, Xiudan Wang 2, Yu Liu 1,3, Zhao Lv 2, Xiaorui Song 1,3, Yiqun Li 2, Lingling Wang 1,3,4 and Linsheng Song 1,3,4,5* Your file should appear on a split screen, with DNA sequence aboveand blank page belowД«) Annotate your sequence by typing in the following information inthe lower screen Your name(s) Select your file by double-clicking on it

#Editseq module of dnastar software#

The goals of this excercise are 1) to familiarize you with the use ofthe software package DNASTAR for desktop-based nucleic acid sequence analysisДў) to use the sequence editing and analysis moduleorf DNASTAR to annotate your sequence, remove vector sequence, translateyour sequence and search genetic databases for entries simialr to yoursequence, andДЈ) to introduce you to the Basic Local AlignmentSearch Tool (BLAST)ДЄ) Importing your DNA sequence into DNASTAR format.ДЎ) Put the disk with your *.rpt file into the floppy drive(A:) of the computerДЈ) Open the EditSeq module of DNASTAR Start

Seqedit.html Desktop Sequence AnalysisSoftware